Astro Bot's most difficult level is locked behind finding all 300 Bots and 120 puzzle pieces. Once you've accomplished that completionist feat, you can create a tower of your 300 bots in the Crash Site near the Golden Monument and attempt the Great Master Challenge: a gauntlet of Astro Bot's most difficult enemies and traps. This video will show a complete successful run and help you nab the elusive bot 301.
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http://dlvr.it/TCyFLl